ABSTRACT
Abstract The aim of this short communication is to describe a case of subfertility and otheranomalies associated with the presence of Mycoplasma spumans and Mycoplasma maculosumin a Bernese Mountain Dog kennel. After the arrival of two dogs from abroad, some fertilitydisorders, such as unsuccessful mating, pregnancy losses and abnormal sperm analysis results,were observed. Two consecutive samplings (vaginal swabs) of three and two bitches with prob-lems, respectively, were performed and M. spumans and M. maculosum were identified by PCRand sequencing. After treatment for 15 days with doxycycline and 9 days with azithromycin,successful pregnancies were achieved and the results of the sperm analyses were reversed.Considering that no other infectious agents causing subfertility problems were detected andthat no management measures or other medication apart from these antibiotics were applied,it was concluded that fertility problems were due to the presence of these two Mycoplasmaspecies.